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Title   사람 말초 혈액내 Large Granular Lymphocyte ( NK 세포 ) 의 Interleukin-2 생산에 관한 연구 ( Interleukin-2 ( IL-2 ) Production by Human Large Granular Lymphocyte ( LGL ) - Enriched Population )
Publicationinfo   1984 Jan; 016(01): 26-34.
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Abstract   Human large granular lymphocytes(LGL) are known to be natural killer(NK) effector cells. NK cell activity is augmented by interferon(IFN) and interleukin-2(IL-2). Furthermore, it was reported that LGL produce IFN-a and IFN-r. We have performed this investigation to demonstrate whether LGL can produce IL-2 or not. LGL-enriched (Fraction I; Fr. I) and LGL-depleted (Fraction II , Fr.II) populations were prepared from human peripheral blood lymphocytes(PBL) on a discontinuous Percoll gradient and stimulated with phptohemagglutinin(PHA)-M for various time periods. The culture supernatants(CM) harvested were tested for IL-2 activity with cultured human PBL. After 48hrs, higher activities were seen in CM produced from Fr. II(Fr. II-CM)than those from Fr I (Fr I-CM). And maximal activity was detected in 72hr-cultured Fr. II-CRI. In contrast, within 24hrs, IL-2 activities in Fr. I-CM were higher than in Fr. II-CM. In these results, it was observed that LGL produce IL- 2 with a peak of productivity occurred early(about 24hr) compared with that occurred later(about 72hr) by T cells. And from the fact that LGL produce IFN and IL-2, we suppose both IFN and IL-2 can act as regul- atory molecules for NK cell activation. Addition of adherent cells(20% of effector cells) was shown to inhibit IL-2 production.
저 자   김광혁(K . H . Kim),남상윤(S . Y . Nam),임수덕(S . D . Lim)